Environment ISA 2006 (or 2004), Virtual Server 2005, same machine
Symptom "Publish Web Site" Wizard succeeds, but the Web site is not accessible.
If you want to Publish a Web Site using the Web Listener on port 80, it simply does not work. I couldn't find an error message - at least there wasn't any in the Event Log. I'm not sure about the Dash board. But since I used ISA 2006 the first time...
Reason Virtual Server 2005 requires IIS installed. IIS installs a Default Web site, that listens on port 80 *surprise*.
Solution Stop the Default Web site in IIS and good to go.